Skip to main content
. 2015 Mar 13;5:9100. doi: 10.1038/srep09100

Figure 1. IL-10 leads to phosphorylation of STAT3 and activates an AIR in macrophages, neutrophils, sDC and mast cells, but not in eosinophils.

Figure 1

(A) Schematic of treatment scheme used in this study. Macrophages, neutrophils, splenic sDC, mast cells and eosinophils were either purified (macrophages, neutrophils, sDC) or derived (mast cells, eosinophils) from mouse tissues, treated with IL-10 for 4 h and then subsequently treated with LPS for a further 4 hours. Upon addition of IL-10 STAT3 is phosphorylated in neutrophils (B), macrophages (C), sDCs (D) and mast cells (E), but not in eosinophils (F). Full-length blots are provided in Supplementary Figure 2. (G) qRT-PCR of the pro-inflammatory cytokines Tnf (TNFa), Cxcl10 (IP10) and Il12b, which are down-regulated when IL-10 is combined with LPS treatment except in eosinophils. Error bars are 95% confidence intervals, significantly down-regulated (p < 0.05) changes between +LPS and +IL-10+LPS are indicated. Genes must first be significantly up-regulated by LPS.