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. 2015 Mar 13;5:9100. doi: 10.1038/srep09100

Figure 4. IL-10 suppression of the LPS-initiated pro-inflammatory response is divergent in the four myeloid cell types.

Figure 4

(A) IL-10 suppressed transcripts (AIR transcripts) were defined as those transcripts declining at least 2 fold. (B) and (C) Transcripts were divided into two categories: (i) AIR transcripts – those transcripts that decline by at least 2 fold after LPS induction in at least one cell type and (ii) ‘not AIR' transcripts that did not decline at least 2-fold after IL-10 treatment. (B) Venn diagram of the IL-10 suppressed genes in the four strongest AIR-responding cell types, macrophages, neutrophils, sDCs and mast cells. (C) Venn diagram of ‘not-AIR' transcripts in the four strongest responding cell types. (D) Cell type-specific AIR transcripts are genuinely cell type-specific. AIR transcripts within the ‘any 2 cell types' category were removed from the analysis, but no other constraints were placed. Boxplot outliers are omitted for clarity (See also Fig S9). Mann-Whitney-U test: *p-value < 0.01 between +LPS and +IL-10/+LPS treatments. (E) Box plots showing the changes in gene expression caused by IL-10 on interleukins, chemokines and Tnf family members. Mann-Whitney-U test: *p-value < 0.05 between +LPS and +IL-10/+LPS treatments. (F) Heatmap of genes suppressed by IL-10 in at least three cell types.