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. 2015 Nov 14;7:98. doi: 10.1186/s13098-015-0098-0

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© Zhao et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of dnRhoA and Y27632 on high-glucose-induced MLC phosphorylation. MLC phosphorylation was not detectable in HUVECs cultured at normal glucose concentration but was significantly increased after stimulation with high glucose. MLC phosphorylation was significantly reduced by blocking RhoA and ROCK activities with dnRhoA and Y27632, respectively. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference. a Western blot assay; b bar graph. *P < 0.01 vs. control group; ^# P < 0.01 vs. high-glucose group