Fig. 2.

Broad detection of simian foamy virus (SFV) antibodies in neotropical primates from Brazil. Upper panel shows seroreactivity of representative neotropical primate plasma samples to the combined NWM SFV antigens from spider monkey (asp Ateles species) and marmoset (cja Callithrix jacchus) cell cultures. Lower panel shows reactivity to crude cell lysate antigens from uninfected canine thymocytes (Cf2Th). Seroreactivity was defined for those specimens with reactivity specific to the diagnostic Gag doublet proteins (p68/p72) in the combined viral antigens. Lanes 1 and 2 are from SFV-infected spider and capuchin monkeys, respectively; lanes 3 and 5 are blanks, lane 4 is an uninfected human control; lanes 6–13 are from Saimiri ustus, Alouatta guariba, Cacajao melanocephalus, Chiropotes species, Leontopithecus chrysomelas, Alouatta seniculus, Saimiri sciureus and Leontopithecus rosalia, respectively