Figure 3. S‐mTFD‐MPPB (red) only inhibited whereas R‐mTFD‐MPPB (blue) enhanced and inhibited GABA_AR‐mediated currents .

Representative notch current traces in HEK293T cells expressing GABA_ARs (α₁β₃γ_2L) showing the effects of S‐mTFD‐MPPB and R‐mTFD‐MPPB on currents elicited by 10 μm GABA (A, B) and 10 mm GABA (C, D). Currents in this notch protocol were obtained using a four‐channel superfusion pipette. In each set of three panels: control is an 8.8 spulse of GABA alone; in the central panels 46 μm R‐ or S‐mTFD‐MPPB was co‐applied for 4 s after 1 s of GABA alone, and Wash was an 8.8 s pulse of GABA after cells were held in the bath solution for 5 min. The green inset in the middle panel of D shows on an expanded current scale the brief enhancement before the onset of inhibition.