Figure 2.

MNs from Mag-null mice are more susceptible to nerve-crush injury-induced apoptosis. P5 Wt and Mag-null mice received a contusion of the left sciatic nerve at mid-thigh level. Mice were killed at P10, and MNs≥100 μm² were quantified in cresyl violet-stained LSC sections using the Fiji software. (a) Representative photomicrographs of LSC sections from Wt and Mag-null mice stained with cresyl violet (Scale bar, 10 μm). (b) Quantification of MNs 5 days after nerve lesion. Data are expressed as the MN count in ipsilateral (crushed) versus contralateral (control) LSC. (*P≤0.05, **P≤0.01, n=4 each group). (c) MNs count in the ipsilateral (crushed) LSC as a function of cell soma size. Data are expressed as the percent of MNs in Mag-null versus Wt mice. (*P≤0.05, n=4 each group)