Figure 2. S. marcescens (S.m.) secretomes inhibit corneal wound healing ex vivo.

LB (mock) (n = 6) and secretomes (n = 6) were added onto wounded corneas and incubated for 48 hours. To observe epithelial defects the corneal tissue was stained with Richardson solution (blue stain). Initial wounds (n = 3) are corneas wounded and stained at end of experiment to determine original wound size. HT = secretome incubated at 95 °C for 10 minutes (n = 6). (a) Representative images of porcine corneas treated with secretomes. (b) Measurements of corneal wounds from ex vivo corneal organ culture. Error bars represent one standard deviation. *p < 0.05 by Tukey’s post hoc analysis.