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. 2015 Sep 14;5:14003. doi: 10.1038/srep14003

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Copyright © 2015, Macmillan Publishers Limited

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(a) LB (mock) and secretomes were added to HCLEs and incubated for 4 hours. HCLEs were stained with Alexa-488 phalloidin for actin (green) and Hoechst 33342 for DNA (blue) and imaged. Scale bar = 10 μm. (b) Actin projections per 30 μm cell area were quantified (LB n = 36, S.m. n = 40). Error bars represent standard deviation. *p < 0.05 by Student’s T test. (c) Stratified HCLEs were treated with LB (mock) and secretomes for 3 hours. Cells were fixed and stained as described above. The center of the “wound” was imaged by confocal microscopy. Scale bar = 50 μm.