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. 2015 Apr 23;6(4):e1734. doi: 10.1038/cddis.2015.103

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Prr14 is upregulated during skeletal muscle differentiation. C2C12 cells were plated in GM. After 24 h, differentiation was stimulated by changing the medium into DM. Cells were harvested at indicated time points and the mRNA and protein level was detected by qRT-PCR (a) and immunoblot (b), respectively. (c) The level of PRR14 protein was quantified. The numbers of relative abundance of both mRNA and protein are showed and presented as the mean±S.D. (n=3). (d) Immunoblot analysis of Prr14 in gastrocnemius skeletal muscle obtained from newborn (n=5), as well as 10-week-old (n=3) mice. (e) The protein levels were quantified and two-tailed student's t-test was used to determine the significance of the difference between the two groups