Figure 2.

Prr14 underexpression impeded myotube formation in C2C12 cells. C2C12 cells were transfected with the indicated siRNA in GM. At 24 h post-transfection, cell media was replaced with DM and cultured for 5 days. (a) Transcripts of Prr14 were detected by qRT-PCR with 18 s as an internal control. The numbers were presented as mean±S.D. (n=3). (b) Phase-contrast images of cells cultured with either GM or DM for 5 days were taken and representative ones are shown. (c) FACS analysis of C2C12 cells as in b was performed using propidium iodide with FACS Canto II and quantitative determination of percentage of cells with multiple nuclei (>4 N) was shown. The data were presented as mean±S.D. (n=3) and statistically analyzed by two-tailed student's t-test. (d) Immunofluorescence analysis of MHC (red) and DAPI (blue) of the indicated C2C12 cells cultured in either GM or DM for 5-day were carried out. Representative images are shown