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. 2015 Apr 23;6(4):e1734. doi: 10.1038/cddis.2015.103

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PRR14 overexpression enhances C2C12 myoblasts differentiation. (a) PRR14 overexpression in stable cell line was confirmed by immunoblot. C2C12-cell lines were plated and cultured in GM for 24 h, and then cell media was replaced with DM and cultured for 4 days. The representative phase-contrast images are shown (b). Percentage of cells with multiple nuclei (>4 N) was quantified by FACS, presented as mean±S.D. (n=3) and statistically analyzed by two-tailed student's t-test (c). The expression of MHC in the indicated C2C12-cell culture in either GM or DM was detected by immunostaining (d). The representative images are shown