Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 23;6(4):e1734. doi: 10.1038/cddis.2015.103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

siRNA-mediated Prr14 depletion is associated with dysregulation of lamin A/C and Hp1α. C2C12 cells were co-transfected with siRNA (10 pmol) and EGFP-N1 empty vector (100 ng). Cells were collected 48 h post-transfection and subjected to immunostaining of lamin A/C (a) or lamin B1 (b) and DAPI. In non-specific control siRNA-transfected cells, lamin A/C was located in either nuclear lamina and cell nucleus, whereas PRR14-siRNA-expressing cells displayed reduced lamin A/C protein level and abnormal nuclear morphology (arrow). (c) The percentage of cells with decreased lamin A/C abundance was determined. The numbers are average of three different fields, and 200 cells were counted for each field. The data are presented as mean±S.D. (n=3). (d) siPrr14 or control siRNA-expressing C2C12 cells were analyzed for mRNA of the indicated genes. The data are presented as mean±S.D. (n=3). (e) The cells, same as in d, were analyzed with immunoblot using the indicated antibodies, and quantifications of WB data are presented as the mean± S.D. (n=3) (f)