Figure 7.

PRR14 binds to and regulates HP1α-mediated regulation of MyoD transcriptional activity. (a) Schematic diagram of the domain structure of PRR14 and the protein fragments used in this study. (b) Mutations were introduced into the HP1α-binding domain and the plasmid was sequenced to confirm the mutations. HEK293T cells were transfected with indicated plasmids. Immunoprecipitation was performed with argarose-conjugated flag-antibody (c–e). C2C12 cells cultured in GM were transfected with MCK promoter-driven luciferase reporter plasmid and pRL-TK in the presence of a combination of different plasmids as indicated. After 12 h, medium was replaced by DM to induce differentiation for 24 h. The MCK promoter activity normalized with pRL-TK was measured by dual-luciferase assay (f, g). The data are presented as mean±S.D. (n=5) and statistically analyzed by two-tailed student's t-test