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. 2015 Apr 23;6(4):e1729. doi: 10.1038/cddis.2015.104

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Caspase-3 knockout cells are more sensitive to DNA-damaging agents. (a) PCR analysis showing both full-length (WT) and targeted caspase-3 allele (C3KO). (b) Protein expression of caspase-3 in HCT116 WT and two independent C3KO clones. Molecular weight markers are indicated on the right. (c) Adherent cells in HCT116 WT and two independent C3KO clones stained by crystal violet 48 h after treatment with camptothecin (500 nM), etoposide (50 μM), or 5-FU (50 μg/ml). (d) Cell proliferation measured by MTS assay in cells as treated in (c). (e) Cell death in cells as treated in (c), including fractions of either PI+ or Annexin V+, or both (PI+/Annexin V+) populations. (d, e) Data are the mean + S.E.M. of triplicate wells. **P<0.01