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. 2015 Apr 23;6(4):e1729. doi: 10.1038/cddis.2015.104

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Formation of a z-VAD-resistant pro-casapse-8 complex after 5-FU treatment. HCT116 or C3KO cells were treated with DMSO or 5-FU (50 μg/ml). (a) Levels of caspase-8 and RIP1 at 24 h. (b) Analysis of caspase-8 (IP-Cas-8) or caspase-3 interacting proteins (IP-Cas-3) at 24 h. Input, whole cell lysate. (c) Analysis of caspase-8 (IP-Cas-8), caspase-3 (IP-Cas-3), or RIP1 (IP-RIP1) interacting proteins. Cells treated as (b) in the presence of z-VAD (20 μM). (d) Reconstitution of catalytically dead (C163A) or full-length caspase-3 (FLC3) in C3KO cells confirmed by western blotting. (e) Levels of HMGB1 in the medium of reconstituted C3KO cells at 48 h. (f) Fractions of PI+/Annexin V- in the reconstituted C3KO cells at 48 h. Data are the mean + S.E.M. of triplicate wells. **P<0.01