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. 2015 Apr 23;6(4):e1729. doi: 10.1038/cddis.2015.104

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RKO caspase-3 KD cells are sensitized to DNA-damaging agents. (a) Stable caspase-3 knockdown (shC3) RKO cells confirmed by western blotting. (b) Crystal violet staining of adherent RKO GFP or shC3 cells 48 h after CPT (500 nM), Etoposide (50 μM) or 5-FU (50 μg/ml) treatment. (c) Levels of HMGB1 in the medium from cells at 48 h with or without 5-FU treatment. (d) Fractions of PI+/Annexin V- cells as treated in (c). (e) ATP levels in cells as treated in (c). (f) Fractions of PI+/Annexin V- cells 48 h after 5-FU treatment, with or without z-VAD (20 μM) or Nec-1 (15 μM). (d–f) The data are mean + S.E.M. of triplicate wells. **P<0.01