Figure 5.
Each Bid chimera, except for BidBad, can activate recombinant Bak to permeabilize liposomes. Nickel-chelating liposomes were treated with 150 nM His-tagged BakΔC and Bid chimeras as indicated and tested for the release of 5(6)-carboxy-fluorescein. Data were normalized by subtracting the signal from untreated liposomes, and then expressed as a percentage of the signal induced by permeabilization with 1% CHAPS. Error bars indicate the S.E.M. of four separate experiments. Statistical significance (‘NS' not significant, **P<0.01, ***P<0.001) was calculated using non-normalized data and a one-tailed Student's t-test