Figure 4.

Reducing basal NF-κB activity upregulates Hgf expression in MDSCs. (a) Real-time RT-PCR analysis revealed that Hgf was significantly upregulated in p65^+/− compared with WT MDSCs (*P<0.05). (b) Hgf transcription could also be induced in WT cells by treatment with an IKKi for 24 h (left). IKKi treatment of p65^+/− MDSCs induced only a modest increase in Hgf at 2 h (*versus vehicle, P<0.05; ⁺versus time=2 h; P<0.05). (c) p-Met in RAW cells can be detected within 5 min of stimulation with HGF (left), or p65^+/− CM (right), but not WT-CM (middle). (d) Inhibiton of Met activation on RAW cells using SU11274 significantly decreased Il6 induction 3 h following stimulation with LPS and p65^+/−-CM, but not LPS and WT-CM. (e) Il10 induction by LPS in both WT-CM or p65^+/− CM was decreased by SU11274, but to a much greater degree in the p65^+/− CM group (*versus vehicle, P<0.05). Values from a representative experiment are displayed as mean±S.E.M. Each experiment was performed at least three times