Figure 5.

Inhibition of caspase-1 reduces photoreceptor cell death after RD. (a) Inhibitor of caspase-1 (YVAD) was administered into the subretinal space, and cleaved IL-1β was measured with western blotting to evaluate the efficiency of caspase-1 inhibition. (b) Densitometry analyses of the western blotting data of (a) normalized to the intensity of β-actin (n=4). (c) TUNEL staining (green) in WT and Rip3^−/− mouse retinal sections. The eyes were treated with vehicle and YVAD and analyzed at 24 h after RD. Nuclei staining: TO-PRO-3 (blue), scale bar: 50 μm. (d) Quantification of TUNEL-positive cells in the ONL after RD. n=4–10 at each groups. (a and b) Data are presented as mean±s.d. *P<0.05, **P<0.01