Figure 4.

p66^Shc depletion increases cell invasion and migration. (a) Boyden chamber assay for A549 cells transduced with control or p66^Shc shRNA as described in Materials and methods. Scale bars, 100 μm. Quantification of invasion change are shown in the right panel. **P<0.01 as compared with the cells treated with control shRNA alone. (b) A549 cells were transduced as in a and plated on fibronectin-coated coverslips. Phase-contrast images were obtained immediately after wounding and at 36 and 72 h after wounding. Quantification of mean speed of cells migrating into the wound shown in the right panel. Error bars represent S.E.M. *P<0.05 as compared with the cells treated with control shRNA alone, based on three independent experiments. (c) A549 p66^Shc-overexpressing cells were plated on fibronectin-coated coverslips. Phase-contrast images were obtained immediately after wounding and at 24 and 48 h after wounding as in b, respectively