Figure 3.

Loss of Wg signaling suppresses Bsk-mediated cell death in other tissues. Light micrographs of Drosophila adult notum (a–e) and wings (f–j), fluorescent micrographs of third instar wing discs (k–o) are shown. Compared with the pnr-Gal4 control (a), ectopic expression of Hep produces a small scutellum phenotype (b), which is partially suppressed by mutations in wg (c), dsh (d), or arm (e). Compared with the ptc-Gal4 control (f), expression of Hep produces a loss-of-ACV phenotype (g), which remains unaffected by expression of LacZ (h), but is suppressed partially by mutation in dsh (i) and strongly by RNAi knocking-down of dsh (j). Compared with the ptc-Gal4 control (k), expression of Hep induced extensive cell death (l), which remains unaffected by expression of LacZ (m), but is suppressed significantly by mutation in dsh (n) and RNAi knocking-down of dsh (o). (p) Statistics of the loss-of-ACV phenotype in (f–j). (f, 0.00%, n=203; g, 77.23%, n=101; h, 86.05%, n=86; i, 43.40%, n=53; j, 6.08%, n=148). Three asterisks, P<0.001; n.s., P>0.05. (q) Statistics of the AO-positive cell number in (k–o). For each genotype, at least 10 discs were analyzed. Three asterisks, P<0.001; n.s., P>0.05