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. 2015 Apr 9;6(4):e1713. doi: 10.1038/cddis.2015.85

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Activation of Bsk signaling promotes wg transcription. Fluorescent micrographs of third instar wing pouches are shown. Compared with the en-Gal4 UAS-GFP controls (a, d), Wg (a) or wg-LacZ (d) expression is significantly elevated by ectopic expression of Egr (b, e) or Hep^CA (c, f) in the posterior compartment (indicated by GFP). For (c) and (f), flies were reared at 18 °C till early third instar larvae, Hep^CA expression was induced at 29 °C for 21 h before dissecting, Wg and wg-LacZ expression is detected by anti-Wg and anti-β-gal antibody