Figure 1.

Effects of spermidine on RGC loss and IRL thickness following ONI. (a) Experimental protocols. Spermidine (30 mM) was added to drinking water for the treatment group from 4 days before ONI throughout the experimental period and the control group received normal drinking water. The treatment period was completed on d14 after ONI. (b) Retinal sections stained with hematoxylin and eosin at d3, 7 and 14 after ONI in control and spermidine-treated mice. Scale bar: 100 and 50 μm in the upper and immediately lower panels, respectively. GCL, ganglion cell layer; INL, inner nuclear layer; IRL, inner retinal layer; ONL, outer nuclear layer. Quantification of the RGC number (c) and IRL thickness (d) in control and spermidine-treated mice. The data are presented as means±S.E.M. of six samples for each experiment. *P<0.05, **P<0.01