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. 2015 Apr 16;6(4):e1720. doi: 10.1038/cddis.2015.93

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effects of spermidine on ONI-induced iNOS activation in the retina. (a) mRNA expression levels of iNOS at d5 after ONI were determined using quantitative real-time PCR. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. The data are presented as means±S.E.M. of six samples for each experiment. *P<0.05. (b) Double-labeling immunohistochemistry of iba1 (green) and iNOS (red) at d5 after ONI. Overlapping immunoreactivities (yellow, arrowheads) indicate the activated microglial cells that produce iNOS. Scale bar: 100 μm