Figure 1.

GATA family members can substitute for OCT4 in pluripotency reprogramming. (A) The reprogramming assay that determines the ability of GATA family members to enhance reprogramming in the absence of OCT4. The Oct4-GFP-positive colonies were counted at 9 days post induction. Induction with empty vector (EV) plus SKM was used as a negative control. Error bars indicate SD (n = 3). (B) Quantitative RT-PCR analysis of the expression of GATA family members, and Nanog in mouse ESCs (R1). R1 cells were cultured in 2i medium. (C) Re-analysis of the expression of GATA family members in early mouse embryo development from published data¹⁵. (D) The generation of iPS colonies with G1SKM (left), G2SKM (middle), and G5SKM (right) from Oct4-GFP MADFs. Phase (upper panel) and GFP images (lower panel) of primary iPS colonies. Scale bar, 500 μm. (E) Germline transmission mice (agouti) from G1SKM (left), G2SKM (middle), and G5SKM (right) are depicted. (F) Quantitative RT-PCR analysis of the expression of endogenous Lhx5 (left) and Dlx3 (right) relative to the expression by SKM induction.