Figure 3.

The GATA family members can activate Sall4 for pluripotency reprogramming. (A) The flow cytometric analysis of GFP in GATA-secondary MEFs. Representative results from three independent experiments are shown. (B) Venn diagram illustrating the overlap between the differentially expressed genes compared with day 0 in GATA4- and GATA6-mediated secondary MEF reprogramming. (C) Quantitative RT-PCR analysis of the relative expression of endogenous Sall4 and Oct4 during GATA-secondary MEF reprogramming. Error bars indicate SD (n = 3). (D) Western blot analysis of SALL4 and OCT4 in GATA-secondary MEF reprogramming. Actin was used as a loading control.