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. 2015 Jan 16;25(2):169–180. doi: 10.1038/cr.2015.6

Figure 4.

Figure 4

Sall4 as a bridge linking the lineage-specifying GATA family to the pluripotency circuit. (A) GATA4 and GATA6 motifs were predicted using the Multiple Em for Motif Elicitation software. ChIP-seq data were generated using GATA4- and GATA6-secondary MEFs. (B) The average ChIP enrichment signals around the TSS of the genes. The red, cyan and purple colors indicate the average ChIP enrichment signals of the top 10%, middle 10%, and bottom 10% of expressed genes from day-6 RNA-seq data. (C) A model for the regulatory interactions of differentially expressed genes reconstructed from binding profiles and expression data. Particular pluripotency-associated factors and epigenetic regulators are highlighted. (D) ChIP-seq binding profiles at the Sall4 and Oct4 loci using secondary MEFs. (E) The flow cytometric analysis of GFP in GATA-secondary MEFs before and after knockdown of the endogenous Sall4 expression. Scrambled shRNA was used as a control. Error bars indicate SD (n = 3).