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. 2015 Jan 23;25(2):237–253. doi: 10.1038/cr.2015.9

Figure 5.

Figure 5

Pulmonary inflammation induced by cationic carriers upon systemic injection in mice. C57BL/6 mice were injected with various carriers: anionic, neutral liposomes (25 mg/kg), cationic DOTAP liposomes (25 mg/kg), PEI (5 mg/kg), Chitosan (25 mg/kg). n = 3 per group. Hematoxylin-eosin (HE) staining (A), immunohistochemistry staining of Gr-1+ cells (B) and specific esterase staining of neutrophils (C) in representative mouse lung sections 24 h after injection are presented. Scale bar, 50 μm (A, C) and 200 μm (B). (D) The stained Gr-1+ cells and neutrophils were counted in ten high power fields (HPFs). (E) Influx of neutrophils in whole lungs was detected after injection with flow cytometry by staining of 7/4 and Ly-6G. Data are mean ± SEM; n = 3.***P <0.001 compared with control group by Student's t-test.