Figure 4.

SPL interacts with CIN-like TCPs and most of them are expressed during ovule development. (A) SPL interacted with CIN-like TCP transcription factors in yeast cells. Transformed yeast cells were spotted on control medium (−2) or selective medium (−3) in 10-fold, 100-fold and 1 000-fold dilutions. The empty vector was used as the control. (B, C) SPL was confirmed to interact with TCP3 (B) or TCP5 (C) in vivo by firefly luciferase complementation imaging assays. (D, E) Overexpression of TCP17-SPLC fusion protein led to the up-curled cotyledons (D) and unfurled flower buds (E) similar to those of SPL overexpression lines. Top of D, schematic representation of 35S-TCP17-SPLC construct. (F-H) The relative expression levels of CIN-like TCPs in the pistils from stage 11 (F) and stage 12 (G), and the mature ovules from stage 13 (H). The expression level of SPL was set to 1.0. The error bars represent the SD of three biological replicates. Scale bars, 1 cm (B, C), 1 mm (D, E), 200 μm (F, G), 50 μm (H).