Figure 1. Cyanamide biosynthesis from L-canavanine.
Panels (A–C) SEM bird eye views of cage-like structures having ~3, 18, and 85 μm2 pore-area. Panel (D) Steps of the fabrication process. From left to right and from top to bottom: (i) a drop of IP-L photoresist is dropcasted into a PDMS chamber previously glued on a glass microscope coverslip, (ii) the resist is exposed by means of TP-DLW, (iii) sample is developed and stored in IPA and (iv) cells are seeded within the chamber. All realized cages were kept in liquid ambient to avoid any deformation induced by solvent evaporation, apart for the samples used for SEM inspection. (Panel E) Representative optical microscope image of a sample after development.