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. 2015 Jun 11;5:11276. doi: 10.1038/srep11276

Figure 2. Antibiotic treatment and co-housing alter the effects of tissue omega-6 and omega-3 PUFA status on metabolic endotoxemia and inflammation.

Figure 2

(a) Schema showing the animal groups and treatments. Separately housed 10-month-old male WT (n = 10) and fat-1 (n = 10) were maintained on the same high n-6 diet, and half in each group received a broad spectrum antibiotic cocktail (ABX) consisting of ampicillin (1 g/L), vancomycin (500 mg/L), neomycin sulfate (1 g/L) (added to drinking water) and metronidazole (100 mg/kg) (orally gavaged every 12 h) for 6 weeks. After treatment, the differences between WT and fat-1 mice in many parameters related to endotoxemia (b) and inflammation (c) were eliminated. For the co-housing experiment, 9-month-old fat-1 (n = 4) and WT littermates (n = 4) were co-housed in two cages (2 mice from each genotype/cage) and fed an identical high n-6 diet after weaning (d). There was no difference between the co-housed WT and fat-1 mice in many parameters related to endotoxemia (e) and inflammation (f), compared to the separately housed WT and fat-1 mice. Data are expressed as mean ± SE. Data with different superscript letters are significantly different (P < 0.05) according to one-way ANOVA with Tukey test.