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. 2015 Jun 19;5:11471. doi: 10.1038/srep11471

Figure 1. Silencing MAP3K3 decreased lung cancer cell proliferation, migration and invasion.

Figure 1

MAP3K3 siRNA (siMAP3K3) was used to knockdown MAP3K3 expression in three lung cancer cell lines (H1299, H838, and HCC78). The non-target siRNA (NT) was used as control. The MAP3K3 expression at the mRNA (A) and protein (The Western blots have been run under the same experimental conditions) (B) levels were decreased more than 90% after MAP3K3 specific siRNA treatment compared to NT. (C): Knocking-down MAP3K3 significantly decreased cell growth at the time point shown compared to NT measured by WST-1. (D): MAP3K3 siRNA inhibited tumor cell migration, and (E): inhibited cell invasion in both H1299 and H838 cells at 72 h. The representative microscopy images are shown on the left (magnification: 10×). The quantification of 10 randomly selected fields is shown on the right. The values shown are expressed as the mean ± SD of three independent experiments. (*p < 0.01; **p < 0.001 versus NT).