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. 2015 Jun 19;5:11471. doi: 10.1038/srep11471

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Copyright © 2015, Macmillan Publishers Limited

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(A): Four phospho-proteins (p-AKTpan, p-GSK3β, p-P38α and p-CREB) were down-regulated after MAP3K3 knockdown at 72 h in both the H1299 and H838 cell lines as compared to non-target siRNA control as determined using the Human MAPK antibody array (The original image is in Supplementary Figure S7), and (B): Quantitation of pixel densities were evaluated using Image J software. (C): The four proteins were then verified by Western blotting (The Western blots have been run under the same experimental conditions), and (D): proteins were decreased ~50% as compared to NT when analyzed by Image J software.