Figure 3. MAP3K3 regulates multiple pathways/genes affecting cell cycle at G₁ arrest and potentially through CDC25A and CCNE1 regulation in lung cancer cells.

Knockdown using MAP3K3 siRNA caused G₁ arrest in both (A) H1299 and (B) H838 lung cells. (C): MAP3K3 regulates many genes in both H1299 and H838 cells, with verification by qRT-PCR (D). (E): DAVID pathway analysis of 235 (206 found in DAVID) down-regulated genes in Fig. 3C showing that these genes are significantly associated with: DNA metabolic, DNA replication and cell cycle processes (p > 6 of –log10). (F): MAP3K3 potentially regulated pathway/genes in lung cancer cells as determined by protein phospho-array and gene expression array analyses in this study. ERK1/2 and ERK5 protein/mRNA were not changed after MAP3K3 siRNA treatment.