Skip to main content
. 2015 Sep 11;25(10):1121–1136. doi: 10.1038/cr.2015.108

Figure 3.

Figure 3

Siglec1 inhibits IRF3 phosphorylation by targeting TBK1. (A) IFN-β luciferase activity in HEK293T cells co-transfected with RIG-I, MAVS, TBK1, IKKɛ, or IRF3(5D) and Siglec1 as indicated. (B) Immunoblot analysis of the indicated molecules in lysates of macrophages transfected with Siglec1 siRNA#1 and infected with VSV (MOI = 10). (C) Immunoblot and RT-PCR analyses were performed to respectively measure TBK1 protein and mRNA levels in macrophages transfected with Siglec1 siRNA and stimulated with poly(I:C) transfection at the indicated time. Actin serves as a loading control. (D) Immunoblot and RT-PCR analyses were performed to respectively measure TBK1 protein and mRNA levels in RAW264.7 cells transfected with increasing doses of Siglec1 plasmid. Data are shown as mean ± SD or representative photographs. **P < 0.01; NS, not significant. See also Supplementary information, Figure S3.