Skip to main content
. 2015 May 27;5:10283. doi: 10.1038/srep10283

Figure 4.

Figure 4

A: PCR analysis of control and Cetn2 MO embryos; there was a clear increase in BMP4 RNA, the disappearance of FGF8 RNA, and no apparent effect on Wnt8 RNA: B: qPCR analysis of Control, Cetn2MO1 and Cetn3MO embryos, injected in both cells of a two cell embryo and harvested at stage 11. C: A similar analysis carried out with the Cetn2MO2. D: qPCR analysis of embryos injected in both cells of a two cell embryo with Cetn2MO1 alone or together with Cetn2-GFP, Cetn3-GFP, or Cetn4-GFP RNAs (RNAs injected at 200 pg/side, total 400 pg/embryo; MOs injected at 10 ng/side, total 20 ng/embryo). While Cetn2-GFP RNA rescued the morphant phenotype Cetn3 or Cetn4 RNAs did not. E: qPCR analysis of embryos injected in both cells of two-cell embryos with Cetn2MO1 alone or together with Noggin RNA (200 pgs/embryo); Noggin reversed the morpholino effect on BMP4 RNA level but not the effect on FGF8 or FGFR1a RNA levels. F: qPCR analysis of embryos injected in both cells of a two cell embryo with Cetn2MO1 alone or together with either Cetn2-GFP or FGF8 RNAs (200 pgs/embryo); Cetn2 RNA rescued the Cetn2 morpholino’s effects on RNA levels, FGF8 reversed the effect on BMP4 RNA, but not the effects on FGFR1a RNAs. Levels of statistical significance indicated single * <0.05, double ** <0.001.