Table 1. Proteins with more than 1.5-folds phosphorylation level change in two maize genotypes response to ABA and osmotic stress.
| UniProt ID | >Protein name | >Sequence of phosphorylati-on peptides | >PhosphoRS-Site Probabilities (>75%) | >Ion score |
Vp5: OS/control |
vp5: OS/control |
Vp5: OS/control |
vp5: OS/control |
>T-test | >Regulation of ABA and osmotic stress for peptides phosphosites | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 1 | 2 | 3 | Averagea | P-Value/FDR | Averagea | P-Value/FDR | |||||||
| B6TE49 | probable receptor-like protein kinase at1g33260-like | gGFsTVYLAsLSSSR | S(4):77.9 | 18 | 2.151 | 2.481 | 3.261 | 0.299 | 0.343 | 0.301 | 2.631 | 0.000/0.000 | 0.314 | 0.000/0.000 | 0.020 | Up-regulated by osmotic stress with ABA-dependent way |
| K7V8B2 | tata-binding protein-associated factor 172-like | sSAGtTPSk | S(1):100.0; T(5):99.9 | 15 | 4.345 | 3.219 | 2.340 | 0.455 | 0.221 | 0.599 | 3.301 | 0.000/0.000 | 0.425 | 0.000/0.000 | 0.044 | Up-regulated by osmotic stress with ABA-dependent way |
| K7TWA4 | regulatory-associated protein of tor 1-like | fRtPPVsPPQHDFLPGLR | T(3):100.0; S(7):100.0 | 13 | 0.232 | 0.362 | 0.490 | 0.232 | 0.622 | 0.455 | 0.361 | 0.000/0.000 | 0.436 | 0.000/0.000 | 0.505 | Down-regulated by osmotic stress with ABA-independent way |
| *C0PLA9 | nodulin-like protein | eEVTEDSENASSSTtALGGsNQDLSSGk | S(20):99.9 | 43 | 1.813 | 1.592 | 1.203 | 0.403 | 0.556 | 0.469 | 1.536 | 0.041/0.057 | 0.476 | 0.000/0.000 | 0.032 | Up-regulated by osmotic stress with ABA-dependent way |
| *B4FBC9 | patellin family protein | aAEADsEEEk | S(6):100.0 | 44 | 2.015 | 2.745 | 2.489 | 0.505 | 0.477 | 0.375 | 2.416 | 0.000/0.002 | 0.452 | 0.000/0.000 | 0.014 | Up-regulated by osmotic stress with ABA-dependent way |
| K7U7E1 | brefeldin a-inhibited guanine nucleotide-exchange protein 1-like | vLENVHQPsFLQk | S(9):100.0 | 17 | 0.409 | 0.378 | 0.312 | 0.789 | 0.400 | 0.543 | 0.366 | 0.000/0.000 | 0.577 | 0.000/0.001 | 0.179 | Down-regulated by osmotic stress with ABA-independent way |
| *K7TWZ6 | clustered mitochondria isoform x1 | qcDVLsPEEYsDEGWQAASmR | S(6):99.6 | 17 | 2.267 | 2.092 | 2.823 | 0.561 | 0.443 | 0.650 | 2.394 | 0.000/0.001 | 0.551 | 0.000/0.001 | 0.008 | Up-regulated by osmotic stress with ABA-dependent way |
| *K7V8M7 | mdr-like abc transporter | qIsINk | S(3):100.0 | 21 | 0.578 | 0.628 | 0.642 | 0.571 | 0.555 | 0.604 | 0.616 | 0.010/0.021 | 0.577 | 0.000/0.000 | 0.175 | Down-regulated by osmotic stress with ABA-independent way |
| *B8A0C6 | phosphatidate phosphatase lpin2-like | eLVPGGEDsGtGSDDEtVNEPEPPAR | S(9):75.0; T(11):75.0; S(13):75.0; T(17):75.0 | 21 | 2.012 | 1.812 | 2.166 | 0.676 | 0.488 | 0.595 | 1.997 | 0.001/0.005 | 0.586 | 0.000/0.001 | 0.003 | Up-regulated by osmotic stress with ABA-dependent way |
| *B4FWX5 | dihydroxy-acid mitochondrial-like | nAMVIVmALGGstNAVLHLIAIAR | S(12):100.0; T(13):100.0 | 16 | 1.803 | 1.503 | 2.071 | 0.602 | 0.613 | 0.593 | 1.792 | 0.008/0.017 | 0.603 | 0.000/0.001 | 0.020 | Up-regulated by osmotic stress with ABA-dependent way |
| *B4FS10 | TPA: hypothetical protein ZEAMMB73_767959 | aAGGDDSGsGGGFNLGGLGGLFAk | S(7):50.0; S(9):50.0 | 25 | 0.538 | 0.617 | 0.476 | 0.605 | 0.922 | 0.409 | 0.544 | 0.004/0.011 | 0.646 | 0.000/0.001 | 0.449 | Down-regulated by osmotic stress with ABA-independent way |
| *B8A0C6 | phosphatidate phosphatase lpin2-like | eLVPGGEDSGtGSDDETVNEPEPPAR | T(11):75.0 | 45 | 1.567 | 1.632 | 2.166 | 0.612 | 0.656 | 0.595 | 1.788 | 0.048/0.060 | 0.621 | 0.001/0.004 | 0.029 | Up-regulated by osmotic stress with ABA-dependent way |
| *K7V792 | splicing factor 3b subunit 1-like isoform x1 | mADADAtPAAGGAtPGATPSGAWDAtPk | T(7):99.7; T(26):100.0 | 26 | 1.786 | 1.565 | 2.019 | 0.666 | 0.612 | 0.671 | 1.790 | 0.000/0.001 | 0.650 | 0.003/0.011 | 0.010 | Up-regulated by osmotic stress with ABA-dependent way |
| *B6U1M6 | transposon protein | lALPLAGGHVtDNDGEGTAERPTk | T(11):93.7 | 11 | 0.513 | 0.491 | 0.578 | 1.612 | 1.499 | 1.480 | 0.527 | 0.005/0.011 | 1.530 | 0.005/0.017 | 0.003 | Up-regulated by osmotic stress, but down-regulated by ABA |
| *B6U0Y9 | atp binding protein | aVQVSPILDGNQtDADSNTAGEEVASR | T(13):96.4 | 52 | 1.512 | 1.492 | 1.557 | 1.647 | 1.590 | 1.558 | 1.520 | 0.004/0.011 | 1.598 | 0.000/0.001 | 0.190 | Up-regulated by osmotic stress with ABA-independent way |
| *B6SP06 | glycine-rich protein 2b | sLNDGDAVEYTVGsGNDGR | S(14):99.9 | 41 | 2.360 | 1.781 | 2.014 | 1.943 | 1.484 | 1.437 | 2.052 | 0.002/0.007 | 1.621 | 0.005/0.017 | 0.034 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *B6TB18 | lipid phosphate phosphatase 3 | eTLNDVESGsAR | S(10):100.0 | 69 | 0.175 | 0.143 | 0.307 | 1.570 | 1.439 | 1.234 | 0.208 | 0.000/0.000 | 1.415 | 0.005/0.016 | 0.014 | Up-regulated by osmotic stress, but down-regulated by ABA |
| K7U2M6 | heat shock protein sti | dVEPEPEAEPmDLtDEEkDR | T(14):100.0 | 11 | 0.353 | 0.212 | 0.495 | 1.523 | 1.533 | 1.484 | 0.353 | 0.005/0.012 | 1.513 | 0.005/0.016 | 0.007 | Up-regulated by osmotic stress, but down-regulated by ABA |
| K7USN0 | 2og-fe oxygenase family protein | aPVMMVAAAPARPmVmASSGTGGGNIsk | S(27):75.0 | 15 | 1.857 | 1.788 | 2.201 | 1.487 | 1.568 | 1.513 | 1.949 | 0.005/0.011 | 1.523 | 0.005/0.016 | 0.091 | Up-regulated by osmotic stress with ABA-independent way |
| *B4FKD1 | nucleoporin nup53-like | eGSPmDGVVQyQQQSPTTPSGQQSQQQk | Y(11):75.0 | 14 | 0.512 | 0.508 | 0.581 | 1.523 | 1.545 | 1.488 | 0.534 | 0.004/0.011 | 1.519 | 0.004/0.016 | 0.002 | Up-regulated by osmotic stress, but down-regulated by ABA |
| B6TCM5 | duf1664 domain family protein isoform 1 | hNMANAVssMTkHLEQVQssLAAAk | S(19):99.6; S(20):99.6 | 26 | 3.516 | 3.110 | 3.123 | 1.539 | 1.496 | 1.700 | 3.250 | 0.004/0.010 | 1.578 | 0.004/0.014 | 0.009 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| Q3MQ01 | autophagy protein 5 | sQEAEQALAsPAEAGFAk | S(10):81.9 | 11 | 1.861 | 1.512 | 1.631 | 1.651 | 1.499 | 1.508 | 1.668 | 0.029/0.043 | 1.553 | 0.003/0.011 | 0.180 | Up-regulated by osmotic stress with ABA-independent way |
| *K7UKU6 | protein decapping 5-like | iGQLNDEPNGYEDDVIEDDEIsPR | S(22):100.0 | 54 | 2.432 | 2.821 | 2.066 | 1.523 | 1.613 | 1.528 | 2.440 | 0.000/0.001 | 1.555 | 0.002/0.009 | 0.045 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *P04711 | phosphoenolpyruvate carboxylase | hHsIDAQLR | S(3):100.0 | 35 | 5.006 | 3.801 | 4.766 | 1.974 | 1.447 | 1.377 | 4.524 | 0.000/0.000 | 1.599 | 0.001/0.006 | 0.011 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *O04014 | tpa:40s ribosomal protein s6 | dRRsEsLAk | S(4):100.0; S(6):100.0 | 14 | 10.27 | 2.875 | 2.829 | 1.467 | 1.641 | 1.828 | 5.324 | 0.000/0.000 | 1.645 | 0.001/0.005 | 0.288 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| C0P2E1 | disease resistance protein rga2-like | aHFPVImLYSFtsTyDVk | Y(15):94.0 | 16 | 3.023 | 3.640 | 4.087 | 1.533 | 1.831 | 1.557 | 3.583 | 0.000/0.000 | 1.640 | 0.001/0.005 | 0.024 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| B7ZYP2 | pentatricopeptide repeat-containing protein at4g22760-like | aGDIPAARAmFEAmPARDVVsWNSMVAGLAk | S(21):80.0 | 14 | 0.389 | 0.455 | 0.299 | 1.678 | 1.723 | 1.523 | 0.381 | 0.000/0.000 | 1.641 | 0.001/0.004 | 0.000 | Down-regulated by osmotic stress with ABA-dependent way |
| K7U3J5 | set domain protein sdg117 | dDTIVcsPVDLSDAcQSGmDR | S(7):92.7 | 12 | 3.544 | 2.809 | 3.211 | 1.812 | 1.578 | 1.593 | 3.188 | 0.000/0.000 | 1.661 | 0.001/0.003 | 0.010 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *K7UKZ7 | transcription elongation factor spt6-like | eScPtLLSFDSDEDNEDIESDAR | T(5):79.0 | 17 | 0.623 | 0.647 | 0.712 | 1.701 | 1.631 | 1.669 | 0.661 | 0.002/0.007 | 1.667 | 0.000/0.003 | 0.001 | Down-regulated by osmotic stress with ABA-dependent way |
| *B4FXH0 | act-domain containing protein kinase family protein | iEDMDSAyDsDASEEGDDDGDDLSVR | Y(8):84.9 | 18 | 0.347 | 0.251 | 0.196 | 1.701 | 1.723 | 1.651 | 0.265 | 0.000/0.000 | 1.692 | 0.000/0.002 | 0.001 | Down-regulated by osmotic stress with ABA-dependent way |
| *K7TW55 | translocase of chloroplast chloroplastic-like | gGNLGPTEAEAETDDGGEEPASGDGEtPASLAAPMPVVESk | T(27):83.3 | 63 | 1.822 | 1.529 | 1.532 | 2.120 | 1.593 | 1.681 | 1.628 | 0.004/0.011 | 1.798 | 0.000/0.001 | 0.130 | Up-regulated by osmotic stress with ABA-independent way |
| M1H548 | arginine serine-rich protein 45-like | rsPsPPPRR | S(2):100.0; S(4):100.0 | 10 | 0.345 | 0.234 | 0.123 | 2.072 | 1.503 | 1.638 | 0.234 | 0.000/0.000 | 1.738 | 0.000/0.001 | 0.008 | Down-regulated by osmotic stress with ABA-dependent way |
| *K7V1A7 | chromatin structure-remodeling complex protein syd-like isoform x4 | aAVVAELFGDATEGGSDQPLPsPR | S(22):94.8 | 26 | 2.084 | 1.824 | 1.508 | 1.562 | 1.624 | 2.127 | 1.805 | 0.011/0.022 | 1.771 | 0.000/0.001 | 0.929 | Up-regulated by osmotic stress with ABA-independent way |
| *B6UEN7 | ubiquitin ligase protein cop1 | aAsAsPQGPAEEGEGPADR | S(3):100.0; S(5):100.0 | 28 | 2.492 | 3.032 | 2.412 | 1.704 | 1.600 | 1.801 | 2.645 | 0.000/0.000 | 1.702 | 0.000/0.001 | 0.063 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *O04014 | tpa: 40s ribosomal protein s6 | sEsLAk | S(1):100.0; S(3):100.0 | 20 | 4.269 | 3.875 | 4.829 | 1.867 | 1.671 | 1.828 | 4.324 | 0.000/0.000 | 1.789 | 0.000/0.000 | 0.009 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *Q8W149 | cell division cycle 5-like | eSQtPLLGGDNPELHPSDFSGVtPR | T(4):99.9; T(23):99.9 | 47 | 1.578 | 2.420 | 1.953 | 2.199 | 1.503 | 1.763 | 1.984 | 0.003/0.009 | 1.822 | 0.000/0.000 | 0.750 | Up-regulated by osmotic stress with ABA-independent way |
| *B8A298 | histone-lysine n- h3 lysine-9 specific suvh1-like | dSESsPQPPIAAPAESGk | S(5):95.5 | 13 | 3.661 | 2.500 | 3.604 | 3.044 | 1.528 | 1.790 | 3.255 | 0.000/0.000 | 2.120 | 0.000/0.000 | 0.086 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| C0P2E1 | disease resistance protein rga2-like | aHFPVImLYsFtstYDVk | S(10):78.0; T(12):78.0; S(13):78.0; T(14):78.0 | 17 | 0.653 | 0.281 | 0.483 | 2.101 | 2.105 | 1.976 | 0.472 | 0.001/0.003 | 2.061 | 0.000/0.000 | 0.006 | Down-regulated by osmotic stress with ABA-dependent way |
| *B6TXK5 | uncharacterized protein LOC100277637 | gPHAstDDEEEEDDDDEDAYEVER | S(5):99.9; T(6):99.9 | 18 | 1.547 | 1.588 | 1.778 | 2.020 | 2.122 | 2.501 | 1.638 | 0.003/0.009 | 2.214 | 0.000/0.000 | 0.017 | Up-regulated by osmotic stress, but down-regulated by ABA |
| *O04014 | tpa: 40s ribosomal protein s6 | skLsAAAk | S(1):100.0; S(4):100.0 | 16 | 9.494 | 10.39 | 11.99 | 2.572 | 2.373 | 2.786 | 10.63 | 0.000/0.000 | 2.577 | 0.000/0.000 | 0.007 | Up-regulated by osmotic stress with ABA-dependent and independent way |
| *P24993 | photosystem ii phosphoprotein | atQtVEDSSRPkPk | T(2):100.0; T(4):100.0 | 15 | 2.148 | 1.578 | 1.557 | 4.661 | 2.294 | 2.004 | 1.761 | 0.002/0.006 | 2.987 | 0.000/0.000 | 0.199 | Up-regulated by osmotic stress, but down-regulated by ABA |
aEach value represents the average of three biological replicas. The average is significant at a p < 0.05 and a false discovery rate (FDR) <0.05 level. Moreover, these peptides whose UniProt ID are signed with * are also significant under FDR <0.01. FDR values attained by Benjamini-Hochberg method were shown in column and were used to adjust p-values (correction for multiple comparisons). These phosphopeptides whose FDR values were signed with delete line ‘—’ were not significant. ‘-’, not measured. T-test is used to identify whether the difference is significant. A T-test value <0.05 is considered to be significant. OS = osmotic stress.