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. 2015 Oct 27;5:15626. doi: 10.1038/srep15626

Table 2. Most abundance phosphoproteins mediated ABA signaling pathways under osmotic stress.

>Protein/peptide sequence >UniProt ID >Protein name >PhosphoRS-Site Probabilities (>75%) >Ion score Vp5: OS/control
vp5: OS/control
Vp5: OS/control
vp5: OS/control
t-test Regulation of ABA and osmotic stress for peptides phosphosites
1 2 3 1 2 3 Averagea P-Value Averagea P-Value
Transporter
 aLGSFRsNA *Q9ATM4 aquaporin pip2-7 S(7):100.0 16 0.656 0.611 0.650 0.762 0.718 0.755 0.639 0.021 0.745 0.028 0.000 Down-regulated by osmotic stress with ABA-dependent way
 aLGsFR Q9ATM4 aquaporin pip2-7 S(4):100.0 17 0.559 0.702 0.728 0.831 0.825 0.824 0.663 0.047 0.827 0.138 0.096 Down-regulated by osmotic stress with ABA-independent way
 aLGsFRsNA Q9ATM4 aquaporin pip2-7 S(4):100.0; S(7):100.0 17 0.436 0.436 0.347 0.862 0.923 0.822 0.406 0.000 0.869 0.153 0.002 Down-regulated by osmotic stress with ABA-dependent way
 lGsSAsFSR *Q9XF58 aquaporin pip2-4-like S(3):97.3; 27 0.494 0.498 0.714 1.192 1.203 1.126 0.569 0.002 1.174 0.221 0.025 Down-regulated by osmotic stress with ABA-dependent way
 xtPLIAGLAVAAtALAGR B6T195 mitochondrial import inner membrane translocase subunit tim14 T(2):100.0; T(13):100.0 13 2.210 2.004 2.451 0.989 1.024 0.719 2.222 0.018 0.911 0.163 0.027 Up-regulated by osmotic stress with ABA-independent way
 rPAsLR B4FZY1 Na+/H+ antiporter S(4):100.0 14 0.641 0.621 0.553 1.032 0.988 1.099 0.605 0.048 1.040 0.880 0.016 Down-regulated by osmotic stress with ABA-dependent way
 gFVPFVPGsPTESsLPLLPGNEN *B4FZY1 Na+/H+ antiporter S(9):91.3 28 2.946 2.823 2.969 1.139 0.936 0.789 2.913 0.000 0.954 0.396 0.003 Up-regulated by osmotic stress with ABA-dependent way
 gQsALGsALGLIsR *B6U6U2 hexose transporter S(3):100.0; S(7):100.0; S(13):100.0 75 1.683 1.692 1.634 1.071 1.048 1.095 1.670 0.026 1.071 0.580 0.003 Up-regulated by osmotic stress with ABA-dependent way
 tQtGSSSNR B6U937 probable sugar phosphate/ phosphate translocator at3g17430-like T(1):80.0 10 6.023 4.674 3.324 0.758 0.813 0.869 4.674 0.000 0.813 0.106 0.041 Up-regulated by osmotic stress with ABA-dependent way
 lSNsFLAITDsFR C0PEW7 vacuolar amino acid transporter 1-like S(11):94.9 29 1.653 1.553 1.653 1.041 1.141 1.241 1.620 0.041 1.141 0.357 0.019 Up-regulated by osmotic stress with ABA-dependent way
 tPLGAAYEPPSAAAGGGGTtPVNIR *C0PLZ2 probable peptide nitrate transporter at5g13400-like T(20):95.8 28 0.522 0.526 0.580 1.392 0.823 1.129 0.543 0.043 1.115 0.400 0.075 Down-regulated by osmotic with ABA-dependent way
 sAsTPR K7U2V8 zinc transporter S(3):97.7 15 1.733 1.930 1.833 0.945 1.113 1.045 1.832 0.007 1.034 0.760 0.000 Up-regulated by osmotic stress with ABA-dependent way
 qSsLNAAGTssMAVLR K7UMX4 solute carrier family facilitated glucose transporter member 8 S(10):97.8 55 1.728 1.821 1.691 0.912 1.121 1.054 1.747 0.018 1.029 0.701 0.005 Up-regulated by osmotic stress with ABA-dependent way
 nsVSsPIMTR Q6UNK5 abc transporter b family member 1-like S(2):76.7 24 2.429 2.169 2.175 1.294 1.330 1.378 2.258 0.001 1.334 0.047 0.013 Up-regulated by osmotic stress with ABA-dependent way
 sSEGVFVGAFLSMSStAVVskFLVEk B6SP24 K+ efflux antiporter 5-like T(16):87.3 17 0.454 0.436 0.424     0.438 0.000   Up-regulated by ABA
 kAssLQR B6SV26 vacuolar amino acid transporter 1-like S(3):100.0; S(4):100.0 19 1.022 0.911 0.920 1.628 1.423 1.682 0.951 0.817 1.578 0.002 0.013 Down-regulated by ABA
 sTGTAAtGGsDAGLEEGk B6UH65 zinc transporter 2 precursor S(1):78.4 10 0.686 0.645 0.665 2.999 3.120 2.973 0.665 0.069 3.031 0.000 0.001 Down-regulated by ABA
 nYLTPFFTsQTDDDNDDDFSQQPQNR B4FS09 sodium hydrogen exchanger 6-like S(9):74.6 12 2.301 2.340 2.201     2.281 0.000   Down-regulated by ABA
 eGSPMDGVVQyQQQSPTTPSGQQSQQQk B4FKD1 nucleoporin nup53-like Y(11):75.0 14 0.512 0.508 0.581 1.523 1.545 1.488 0.534 0.005 1.519 0.004 0.002 Up-regulated by osmotic stress, but down-regulated by ABA
 tsDADsEAGSGSGGGGR C0P5C4 abc1 family protein S(2):95.0 16 0.701 0.774 0.787 1.660 1.594 1.612 0.754 0.197 1.622 0.001 0.003 Down-regulated by ABA
Ubiquitin-conjugating enzyme family protein-like
 ntPSmPPAVSTSSAsR B4FHK6 ubiquitin-conjugating enzyme e2 22-like T(2):76.1 11 0.456 0.511 0.405 0.782 0.790 0.757 0.457 0.000 0.776 0.053 0.004 Down-regulated by osmotic stress with ABA-dependent way
 eVNAGIASVsR *B4FAG8 e3ubiquitin-protein ligase rhf2a-like isoform x1 S(10):100.0 30 0.605 0.589 0.646 0.912 0.800 0.845 0.613 0.028 0.852 0.196 0.020 Down-regulated by osmotic stress with ABA-dependent way
 rHsTGQstPDR *B4FAG8 e3ubiquitin-protein ligase rhf2a-like isoform x1 S(3):97.0; T(8):97.0 28 2.211 1.890 2.017 1.401 1.201 1.303 2.039 0.002 1.302 0.057 0.002 Up-regulated by osmotic stress with ABA-dependent way
 aDsPSEGLTcGSQNLPAETcPk *K7V4D9 e3ubiquitin-protein ligase upl4-like S(3):99.8 23 2.888 2.521 2.666 1.010 0.987 0.949 2.692 0.000 0.982 0.665 0.003 Up-regulated by osmotic stress with ABA-dependent way
 sAsPSTS C0P3H1 Ubiquitin carboxyl-terminal hydrolase isozyme l5-like S(3):96.4; 16 0.555 0.512 0.597 0.957 0.879 1.020 0.555 0.006 0.952 0.704 0.002 Down-regulated by osmotic stress with ABA-dependent way
 lGVDVNtmPAItDk B4G0Z1 e3ubiquitin-protein ligase ubr7-like T(12):99.6 11 0.601 0.499 0.558 1.230 1.110 1.192 0.553 0.001 1.177 0.221 0.000 Down-regulated by osmotic stress with ABA-dependent way
 aAsAsPQGPAEEGEGPADR *B6UEN7 ubiquitin ligase protein cop1 S(3):100.0; S(5):100.0 28 2.492 3.032 2.412 1.704 1.600 1.801 2.645 0.010 1.702 0.001 0.063 Up-regulated by osmotic stress with ABA-dependent and independent way
 dVsNAsELATEMQYER *K7TFK8 e3ubiquitin-protein ligase upl1-like S(3):100.0; S(6):100.0 28 2.132 1.929 1.958 1.153 1.099 0.900 2.007 0.009 1.051 0.473 0.005 Up-regulated by osmotic stress with ABA-dependent way
 lRPGQPDAVQDAStSDmEDASTSSGGQR *K7TFK8 e3ubiquitin-protein ligase upl1-like T(14):76.0 41 1.656 1.626 1.701 1.420 1.766 1.087 1.661 0.029 1.424 0.020 0.390 Up-regulated by osmotic stress with ABA-independent way
 eNEGSSSsAGESSSmDIDk *B6T6V5 ubiquitin carboxyl-terminal hydrolase 6-like S(8):79.5 17 0.612 0.584 0.658 1.312 1.113 1.215 0.618 0.029 1.213 0.169 0.008 Down-regulated by osmotic stress with ABA-dependent way
 sALLsYSDTVR B6T6V5 ubiquitin carboxyl-terminal hydrolase 6-like S(5):75.0 13 0.580 0.592 0.512 1.799 1.064 1.200 0.561 0.008 1.354 0.048 0.070 Down-regulated by osmotic stress with ABA-dependent way
Zinc finger transcription factor
 dLVVDtDDGGNANR *B6UB08 zinc finger protein 652-a- partial T(6):100.0 46 0.570 0.521 0.564 0.809 0.812 1.089 0.552 0.006 0.903 0.101 0.057 Down-regulated by osmotic stress with ABA-independent way
 dPSINQVAsPVAAPEPVGAILPk *B4FX96 Zinc finger ccch type domain containing protein zfn-like 1 S(9):100.0 26 0.655 0.776 0.516 1.016 0.877 0.769 0.649 0.047 0.887 0.358 0.087 Down-regulated by osmotic stress with ABA-independent way
 eQGsIGITANDDPyNGNEmSPSDQR K7UHH6 zinc finger c-x8-c-x5-c-x3-h type family protein S(4):100 40 0.245 0.360 0.495 0.808 0.904 1.007 0.367 0.000 0.906 0.443 0.001 Down-regulated by osmotic stress with ABA-dependent way
 dPAVGsSPAVsNNk *B4FLK4 zinc finger protein 207-like isoform x1 S(6):97.3 41 0.431 0.423 0.454 1.277 1.292 1.311 0.436 0.000 1.293 0.086 0.000 Down-regulated by osmotic stress with ABA-dependent way
 dWNQNFEVsPTDYLPQDSR *B6U194 zinc finger c-x8-c-x5-c-x3-h type family protein S(9):80.0 12 0.401 0.393 0.536 0.901 0.993 0.821 0.443 0.000 0.905 0.411 0.038 Down-regulated by osmotic stress with ABA-dependent way
 lQPADsIEGTVIDRDcDEVDDAAQDSGAR *B4FY62 tpa:c3hc zinc finger-like family protein S(6):99.9 18 0.422 0.493 0.412 1.112 1.030 0.980 0.442 0.000 1.041 0.891 0.006 Down-regulated by osmotic stress with ABA-dependent way
 dcDEVDDAAQDsGAR *B4FY62 tpa:c3hc zinc finger-like family protein S(12):100.0 35 2.981 1.879 2.006 1.355 1.439 1.402 2.288 0.001 1.399 0.017 0.139 Up-regulated by osmotic stress with ABA-independent way
 cMVsLsPPPPk K7UE59 ring zinc finger domain superfamily protein S(4):100.0; S(6):100.0 21 0.498 0.556 0.587 1.332 1.376 1.385 0.547 0.002 1.364 0.020 0.000 Down-regulated by osmotic stress with ABA-dependent way
 gANEEVsSINVDEDPNVPYERsPNAAIAk *K7UBL3 zinc finger c-x8-c-x5-c-x3-h type family protein S(7):95.1; S(22):100.0 35 0.409 0.627 0.570 0.925 1.328 1.624 0.535 0.002 1.293 0.131 0.041 Down-regulated by osmotic stress with ABA-dependent way
 dSSANPPPsPGTTYGPVGSISk *B6SW01 zinc finger ccch type domain-containing protein zfn-like 3 S(9):83.6 16 0.534 0.424 0.624 0.980 0.883 1.039 0.527 0.005 0.967 0.698 0.001 Down-regulated by osmotic stress with ABA-dependent way
 lGGsDGNsEDDMDNDk *B7ZXU2 serrate-related c2h2 zinc-finger family protein S(4):100.0; S(8):100.0 29 1.694 1.721 1.417 1.220 1.179 1.274 1.611 0.048 1.224 0.151 0.088 Up-regulated by osmotic stress with ABA-independent way
 ePGEGtSS B6TD33 zinc finger ccch domain-containing protein 11-like T(6):83.3 17 1.594 1.623 1.856 1.226 1.112 1.345 1.691 0.027 1.228 0.149 0.010 Up-regulated by osmotic stress with ABA-dependent way
 nVDVDsDGER *K7UZK2 zinc finger ccch domain-containing protein 44-like S(6):100.0 26 2.282 1.968 2.223 1.365 1.063 1.211 2.158 0.001 1.213 0.192 0.001 Up-regulated by osmotic stress with ABA-dependent way
 vEsSLVGSDDVLDSASDSPPsVk C0P2B1 phd zinc finger S(21):74.8 15 2.334 2.410 2.309 1.350 1.597 1.441 2.351 0.000 1.463 0.008 0.003 Up-regulated by osmotic stress with ABA-dependent way
 nTHPPEPESIDGINDtGVQTPQQFR *B4FZ17 dhhc-type zinc finger domain-containing protein T(16):49.0 13 3.911 3.890 3.589     3.797 0.000   Down-regulated by ABA
 eQGsIGItANEDPYNANEMSPSDQR *B4FX77 zinc finger c-x8-c-x5-c-x3-h type family protein S(4):100.0; T(8):94.7 22 1.372 1.375 1.489 0.650 0.580 0.630 1.412 0.143 0.620 0.001 0.002 Up-regulated by ABA
 vPQDEEESGDDDEDEEADEHNNtLcGTcGTNDSk B6TG72 phd finger protein T(27):32.2 10 1.320 1.344 1.462 0.431 0.660 0.778 1.375 0.162 0.623 0.001 0.008 Up-regulated by ABA
 sQPPDAAASPDASIssPSSLGGGGGDAADADAIEk *K7UCK7 zinc finger ccch type domain-containing protein zfn-like 6 S(15):79.2 22 0.998 0.899 1.031 0.656 0.645 0.663 0.976 0.976 0.655 0.000 0.011 Up-regulated by ABA
Ribosomal protein
 gQAAATAsk *B4FCE7 60s ribosomal protein l2 S(8):100.0 33 0.649 0.633 0.624 0.912 0,847 0.967 0.635 0.049 0.940 0.458 0.018 Down-regulated by osmotic stress with ABA-dependent way
 asAAtSA *O04014 tpa: 40s ribosomal protein s6 S(2):100.0; T(5):100.0 29 2.017 1.865 1.947 0.992 1.254 0.913 1.943 0.005 1.053 0.920 0.024 Up-regulated by osmotic stress with ABA-dependent way
 vsEELR *O04014 tpa: 40s ribosomal protein s6 S(2):100.0 16 1.773 1.541 1.476 0.981 0.989 1.121 1.597 0.044 1.030 0.358 0.046 Up-regulated by osmotic stress with ABA-dependent way
 fTADDVAAAAGGAAAtGAsLQEID *B6TPG2 60s ribosomal protein l26-1 T(16):100.0; S(19):100.0 20 0.456 0.387 0.523 1.301 1.299 1.286 0.455 0.000 1.295 0.075 0.003 Down-regulated by osmotic stress with ABA-dependent way
 eEsDDDMGFSLFD *B6UE07 60s acidic ribosomal protein p2a S(3):100.0 44 1.920 2.079 1.631 1.013 0.995 1.174 1.877 0.003 1.061 0.261 0.049 Up-regulated by osmotic stress with ABA-dependent way
 fASVPcGGGGVAVAAAsPAAGGAAPTAEAk *B6UE07 60s acidic ribosomal protein p2a S(17):80.0 27 0.498 0.487 0.536 1.371 1.104 1.240 0.507 0.002 1.238 0.127 0.010 Down-regulated by osmotic stress with ABA-dependent way
 kAsGGGGDDEEEE B4FCK4 40s ribosomal protein s9 S(3):100.0 19 0.987 1.082 1.020 1.560 1.487 1.550 1.030 0.643 1.532 0.004 0.010 Down-regulated by ABA
 eEEkAPEPAEEsDEEMGFSLFDD *B4FWI0 60s acidic ribosomal protein p0 S(12):100.0 12 1.752 1.687 1.681     1.706 0.000   Down-regulated by ABA
WD-40 repeat protein
 vSNNDSEPDsPSGSPNR *B6TM01 transducin wd-40 repeat S(10):100.0 49 1.608 1.264 1.921 1.070 0.804 1.020 1.598 0.022 0.965 0.801 0.043 Up-regulated by osmotic stress with ABA-dependent way
 gRSsPVVsGSPSQNSDGSmsSWR B4FMI7 wd repeat-containing protein 89 homolog S(20):83.9 17 1.221 1.262 1.276 1.776 1.660 1.676 1.253 0.321 1.704 0.000 0.013 Down-regulated by ABA
 ssPVVsGSPSQNSDGSmSSWR B4FMI7 wd repeat-containing protein 89 homolog S(2):96.0 12 3.083 3.880 3.912 1.240 1.022 1.012 3.625 0.000 1.091 0.701 0.018 Up-regulated by osmotic stress with ABA-dependent way
Arginine serine-rich splicing factor
 gNNGDDEHRGsPRGsQsP C0HIN5 arginine serine-rich splicing factor rs2z37a transcript i S(11):100.0; S(15):100.0; S(17):100.0 15 0.336 0.301 0.485 0.756 0.801 0.785 0.374 0.000 0.781 0.063 0.020 Down-regulated by osmotic stress with ABA-dependent way
 sEGSSSsSFGR *B7ZYN1 serine arginine repetitive matrix protein 2-like S(7):79.6 27 0.601 0.552 0.705 0.850 0.798 0.844 0.620 0.038 0.831 0.190 0.028 Down-regulated by osmotic stress with ABA-dependent way
 eRsPGAR B6SY05 arginine serine-rich splicing factor rsp41 S(3):100.0 17 1.680 1.781 2.356 0.878 0.880 0.825 1.939 0.008 0.861 0.218 0.042 Up-regulated by osmotic stress with ABA-dependent way
 gGtPPR K7V1I2 arginine serine-rich splicing factor sr45_2 transcript i T(3):100.0 18 0.310 0.308 0.387 0.859 0.949 0.827 0.335 0.000 0.878 0.234 0.011 Down-regulated by osmotic stress with ABA-dependent way
 qYRsPsADR K7U6X8; B4FD63 serine arginine repetitive matrix protein 2-like isoform x2 S(4):99.9; S(6):100.0 11 0.626 0.504 0.613 0.866 0.945 0.941 0.581 0.017 0.917 0.616 0.029 Down-regulated by osmotic stress with ABA-dependent way
 aAcsGsP *M1GS93 splicing arginine serine-rich 2 S(4):100.0; S(6):100.0 26 0.555 0.564 0.560 0.849 0.835 0.916 0.559 0.011 0.867 0.199 0.007 Down-regulated by osmotic stress with ABA-dependent way
 sYTPDDINDR *B4FQ73 serine arginine-rich splicing factor 33-like S(1):100.0 11 1.789 1.954 1.864 0.977 1.010 0.945 1.869 0.007 0.977 0.654 0.002 Up-regulated by osmotic stress with ABA-dependent way
Heterogeneous nuclear ribonucleoprotein
 ssQGGGGYR C0P8S9 heterogeneous nuclear ribonucleoprotein a2 S(2):100.0 12 0.597 0.567 0.685 0.812 0.845 0.815 0.616 0.029 0.824 0.114 0.040 Down-regulated by osmotic stress with ABA-dependent way
 sPAGGQNYAmSR *B8A134 heterogeneous nuclear ribonucleoprotein 1-like S(1):100.0 12 0.587 0.543 0.618 0.897 0.834 0.847 0.583 0.015 0.859 0.202 0.008 Down-regulated by osmotic stress with ABA-dependent way
 lGsPIGYVGLNDDSGSILSSMSR *B8A134 heterogeneous nuclear ribonucleoprotein 1-like S(3):95.1 13 2.221 2.340 2.179 0.987 1.021 1.163 2.247 0.001 1.057 0.289 0.006 Up-regulated by osmotic stress with ABA-dependent way
 qPsEEPEEQVDLEGDDDGmDDDDAGYR *K7UBY5 heterogeneous nuclear ribonucleoprotein r-like S(3):100.0 74 1.789 1.801 1.894 0.867 0.887 1.080 1.828 0.010 0.945 0.845 0.002 Up-regulated by osmotic stress with ABA-dependent way
 rGsRDDsEEPEEDDDNDER *K7UBY5 heterogeneous nuclear ribonucleoprotein r-like S(3):100.0; S(7):100.0 29 1.923 2.088 2.265 1.031 1.176 0.963 2.092 0.002 1.057 0.880 0.016 Up-regulated by osmotic stress with ABA-dependent way
 dDsEEPEEDDDNDER *K7UBY5 heterogeneous nuclear ribonucleoprotein r-like S(3):100.0 55 1.577 1.779 2.056 1.208 1.302 1.436 1.804 0.002 1.315 0.880 0.021 Up-regulated by osmotic stress with ABA-dependent way
 eANPGGsGGGR B8A305 heterogeneous nuclear ribonucleoprotein 1-like isoform x1 S(7):100.0 11 0.565 0.612 0.671 1.210 0.988 1.102 0.616 0.031 1.100 0.508 0.028 Down-regulated by osmotic stress with ABA-dependent way

OS = osmotic stress.

aEach value represents the average of three biological replicas. A p value <0.05 in regard of FDR <0.05 is considered to be significant. Nevertheless, these peptides whose UniProt ID are signed with * are also significant under FDR <0.01. ‘-’, not measured. T-test is used to identify whether the difference is significant. A T-test value <0.05 is considered to be significant.