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. 2015 Jun 3;5:10870. doi: 10.1038/srep10870

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Copyright © 2015, Macmillan Publishers Limited

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(a) F-actin stabilization was examined using a high-speed cosedimentation assay. A mixture of depolymerizing F-actin in the absence or presence of VopV_rep1, latrunculin B, and phalloidin was ultracentrifuged after 0, 2, or 4 h incubation. The pellet fractions (F-actin) were separated using SDS-PAGE, and the gels were stained with Coomassie blue. (b) The stabilized F-actin shown in (a) was analyzed using densitometry. The relative F-actin ratio was calculated as the amount of F-actin pelleted at each time point divided by that of zero time point amount.