Table 1. HBV DNA methylation in various HBV-infected livers by BS-PCR sequencing.
| Percent of methylated CpG sites€ | CpG island | ||
|---|---|---|---|
| 1 | 2 | 3 | |
| Hepatitis | 6.6% (5/76) | 6.0% (15/250) | 17.5% (16/91) |
| Cirrhosis | 0% (0/60) | 0.8% (2/236) | 10.8% (7/65) |
| HCC | 16.1% (20/124) | 8.0% (32/398) | 52.5% (75/143) |
| p value# HCC vs. (Hepatitis and Cirrhosis) | 0.0007 | 0.0046 | <0.0001 |
€The percent of methylated CpG sites was calculated as the number of methylated CpG sites detected / total CpG sites with valid BS sequencing data (methylated + unmethylated) shown in Fig. 2.
#Fisher’s two-tailed exact test.