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. 2015 May 28;5:10473. doi: 10.1038/srep10473

Figure 3.

Figure 3

Interstrand cross-linking reactions of two duplexes ODN1/ODN4 and ODN2/ODN4. Cross-linking conditions: [ODN1 or ODN2] = 10 μM, ODN4 = 1 μM, 10 mM PBS buffer, 100 mM NaCl, 37 °C, pH 7.0. The reaction was followed by electrophoresis using 20% denatured polyacrylamide gel. The fast- and slow-moving bands represent ODN4 and the cross-linked products, respectively. The cross-linking yields were obtained by quantification of the bands by FAM fluorescence, and they were then plotted against time. (a) Yields (%) obtained with ODN1 and ODN4-FAM (X = T, G, C, A). (b) Gel-shift analysis of the reaction with ODN1. (c) Yields (%) obtained with ODN2 and ODN4-FAM (X = T, G, C, A). (d) Gel-shift analysis of the reaction with ODN2. CON =control.