Figure 5.

Cross-linking reaction with ODN3 and ODN9. Cross-linking conditions: ODN3 = 10 μM, ODN9 = 1 μM, 10 mM PBS buffer, 100 mM NaCl, 37 °C, pH 7.0. The reaction was followed by electrophoresis using 20% denatured polyacrylamide gel. The fast- and slow-moving bands represent ODN4 and the cross-linked products, respectively. The cross-link yield was obtained by quantification of the bands by FAM fluorescence, and it was plotted against time. (a) Yields (%) obtained with ODN3 and ODN9. (b) Gel-shift analysis of the reaction with ODN3. CON =control.