Table 4. Expression of some genes induced by WSHM detected by Real Time PCR.
| Expression* (×10−2) induced by |
||||
|---|---|---|---|---|
| Spot No. | Protein | Genistein | Control | WSHM |
| 39 | nitrogen regulatory protein PII | 1.14 ± 0.0100b | 0.220 ± 0.0600c | 3.76 ± 0.160a |
| 209 | DNA-directed RNA polymerase, beta subunit | 6.35 ± 0.0500b | 3.10 ± 0.0300b | 59.4 ± 3.11a |
| 395 | isopropylmalate isomerase small subunit | 6.26 ± 0.0800b | 1.52 ± 0.0600c | 30.7 ± 2.22a |
| 1858 | putative phosphoribulokinase protein | 86.4 ± 3.99a | 20.4 ± 0.270c | 58.7 ± 1.42b |
| 1859 | ABC transporter substrate-binding protein | 1.95 ± 0.210b | 1.74 ± 0.510b | 39.8 ± 0.800a |
| 1860 | ATP synthase FliI/YscN | 0.610 ± 0.0400b | 0.250 ± 0.0200c | 2.47 ± 0.460a |
Assays were performed in triplicate, and the mean values and SDs are shown. Values in the same line having different letters are significantly different from each other according to LSD test (P < 0.05).
*Fold change in gene expression (vs 16s rDNA).