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. 2015 Jul 27;5:10642. doi: 10.1038/srep10642

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VDR binds to the Claudin-2 promoter. (A) CHIP-PCR amplification demonstrated binding of VDR to the promoter regions of human Claudin-2 in SKCO15 cells. PCR were performed including input-positive controls and IgG/villin-negative controls. n = 3 separate experiments. (B) ChIP-PCR assays of VDR binding to the Claudin-2 promoter in mouse colon. n = 3 separate experiments. (C) PCR analysis of RXR in VDR^+/+ or VDR^-/- mice. Data are expressed as mean ± SD. *P < 0.05. n = 3 separate experiments.