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. 2015 Jun 15;5:11433. doi: 10.1038/srep11433

Figure 2. Effect of exogenous trehalose on ROS accumulation in leaves of rice seedlings with or without Cu stress.

Figure 2

(a) superoxide (O2∙−) and (b) hydrogen peroxide (H2O2) production in rice leaves were detected using nitroblue tetrazolium (NBT) solution and 3,3'-diaminobenzidine (DAB), respectively, at day 7 of Cu stress. Control, Tre, Cu, and Tre+Cu correspond to control, 10 mM trehalose, 100 μM CuSO4, and 10 mM trehalose + 100 μM CuSO4, respectively.