Table 2. The amplifying and QuikChange primers used in this study with mutated amino acids in the sense primers underlined.

Primers	Sense Primers	Antisense Primers
EcEndoV-FL	5′-GATAGGGCCATATGGATCTCGCGTCATTACGCG-3′	5′-TTGCACTTCTCGAGTCAGGGCTGATTTGCTGTATAGC-3′
EcEndoV-SF	5′-GATAGGGCCATATGGATCTCGCGTCATTACGCG-3′	5′-TTGCACTTCTCGAGTCAGGCCACCGCGTCCGCCCAG-3′
EcEndoV-FL-Y73F	5′-TACCACCATGCCTTTCATTCCAGGTTTTC-3′	5′-GAAAACCTGGAATGAAAGGCATGGTGGTA-3
EcEndoV-FL-D183R	5′-CATCGGGTCAGCGTGCGCAGCGCGCTGGCGTG-3′	5′-CACGCCAGCGCGCTGCGCACGCTGACCCGATG-3′
EcEndoV-FL-S144A	5′-CGAACCGCTCTCCGCCGAACCGGGCGCG-3′	5′-CGCGGCCCGGTTCGGCGGAGAGCGGTTCG-3′
EcEndoV-FL-E140R	5′-TATGCGGTAAATTCCGACCGCTCTCCAGCG-3′	5′-CGCTGGAGAGCGGTCGGAATTTACCGCATA-3′
ssDNA-I	5′-CTGATCGICGATCAG-3′
ssDNA-A	5′-TTCTCCCGCAGCTGC-3′
ssDNA-B	5′-GTGGTCCATATCCACGCTAG-3′
ssDNA-C	5′-ACGATTTGCAACTATTCGAACTCCT-3′
ssDNA-D	5′-CCGGGATCAAGTCCACTCACTTAGATGCAC-3′
ssRNA-I	5′-CUGAUCGICGAUCAG-3′