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. 2015 Jul 2;6(7):e1802. doi: 10.1038/cddis.2015.150

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HOTAIR-promoted gastric cancer cell invasion, metastasis and EMT. (a) qRT-PCR was used to detect HOTAIR expression of BGC-823 and SGC-7901 cells with si-HOTAIRs. (b) Transwell assays were used to investigate the changes in migratory and invasive abilities of GC cells. (c) The lungs from mice in each experimental group with the numbers of tumor nodules on lung surfaces and weight were shown. (d) Visualization of the entire lung and HE-stained lung sections. (e and f) Western blotting and qRT-PCR were performed for analysis of E-cadherin, N-cadherin and vimentin in BGC-823 and SGC-7901 cells with si-HOTAIR. All experiments were performed in triplicate with three technical replicates. *P<0.05 and **P<0.01