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. 2015 Jul 2;6(7):e1801. doi: 10.1038/cddis.2015.155

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Higher expression of FoxM1 is found in CD44⁺ HCT-15 cells, which are more sensitive to thiostrepton. (a) Protein levels of CD44 and FoxM1 in CD44⁺ and CD44⁻ HCT-15 cells were analyzed by western blotting. Tubulin signal was used as a loading control. (b) The IC₅₀ values of thiostrepton on CD44⁺ and CD44⁻ HCT-15 cells were determined as described. *P<0.05 by Student's t-test. (c) A colony-formation assay was carried out by treating cells with DMSO (Veh) or 5 or 10 μM thiostrepton for 6 h before their washout of drug or control. Cells were cultured in regular media for 10 more days and colonies were stained with crystal violet. Data representing the mean±S.D. of three independent experiments were analyzed by Student's t-test (*P<0.05)