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. 2015 Jul 2;6(7):e1801. doi: 10.1038/cddis.2015.155

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Knockdown of FoxM1 expression in HT-29 and HCT-116 cells reduces their CD44⁺ subpopulations and their sensitivity to thiostrepton. Vector-control and FoxM1-knockdown clones were established from HT-29 (29 V, 29sh1 and 29sh2) and HCT-116 (116 V, 116sh1, and 116sh2) cells by retroviral infection. (a) The protein levels of FoxM1 and CD44 in the aforementioned clones were analyzed by western blotting. Actin signal served as a loading control. (b) The percentage of CD44⁺ subpopulations (numbers in the top right quadrant) in 29 V and 29sh1 clones and 116 V and 116sh1 cells were measured by flow cytometry. (c) The IC₅₀ values of thiostrepton on the aforementioned four clones were determined as described in the ‘Materials and Methods'. *P<0.05 when compared with their respective vector control clones by Student's t-test