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. 2015 Jul 16;6(7):e1818. doi: 10.1038/cddis.2015.182

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Reduction of AIP1 level and increase of F-actin level as the results of Aip1 deletion in either Sertoli cells or germ cells. (a and b) Western blot analysis of AIP1 protein levels in isolated THY1+ germ cells from geAip1^−/− testes at P7 (a) and isolated Sertoli cells from seAip1^−/− testes at P7 (b). The control for (a) is THY1+ germ cells from Aip1^fl/+ testes and it is compared with THY1+ germ cells from geAip1^−/−. The control for (b) is Sertoli cells from Aip1^fl/fl testes and it is compared with Sertoli cells from seAip1^−/− (c and d) Representative confocal images of phalloidin staining (red) of tissue sections from the P7 controls, geAip1^−/− (c) and seAip1^−/− (d) testes. Cell nuclei were stained with DAPI (blue). Scale bars: 10 μm